Faculty Interest Inventory
Office for Research
Mon, 2011-03-14 14:46 — daig
Center for Biotechnology and Interdisciplinary Studies (CBIS)
Dr. Dai graduated from Beijing University, China with B.S. in Mechanical Engineering and M.S. in Biomechanics, where he performed research on cardiovascular system modeling and the dynamic coupling of left ventricle and systemic arteries. After that, he came to Massachusetts Institute of Technology and subsequently joined the Harvard-MIT Division of Health Science’s Medical Engineering and Medical Physics program. During his Ph.D. studies, he did research in Fluid Mechanics Laboratory at MIT and Vascular Surgery Research Laboratory at Massachusetts General Hospital. There, he developed a biomechanical model to analyze venous blood flow and tissue mechanics in the lower leg, and optimized the design of external pneumatic compression (EPC) device for better deep vein thrombosis (DVT) prophylaxis. Then, he completed Post-doctoral training in Dr. Michael Gimbrone’s laboratory (Center for Excellence in Vascular Biology) at Harvard Medical School. His research focuses on the influence of biomechanical force on endothelial phenotypic modulation and its role on pathogenesis of atherosclerosis. He has developed an in vitro system to recreate arterial shear stress waveforms acquired from atherosclerosis-susceptible and -resistant regions of human vasculature, and studied endothelial functions under these conditions. Using this system, combined with genome-wide transcriptional profiling strategies, his work has revealed distinct global gene expression patterns and some of the underlying molecular mechanisms that are responsible for the disease-prone and –protected phenotypes of vascular endothelium.
Dr. Dai’s education and research experiences concentrate in the field of cardiovascular biomechanics and vascular biology. He is a member of Biomedical Engineering Society and North American Vascular Biology Society. Dr. Dai serves as a reviewer for National Science Foundation, American Heart Association, Journal of Biomechanical Engineering, American Journal of Physiology, Cell and Molecular Bioengineering, Cardiovascular Bioengineering, Tissue Engineering and Journal of Vascular Surgery.
Vascular endothelium plays an increasingly important role in many physiological and pathological processes in cardiovascular system. The functional phenotypes of the vascular endothelium are constantly modulated by its surrounding environments, including interactions with blood components, smooth muscle cells, extracellular matrix and biomechanical forces. Dysfunctional endothelium can contribute to the pathogenesis of many vascular diseases such as inflammation, thrombosis, atherosclerosis and failure of vascular graft. The mission of Vascular Bioengineering Laboratory is to integrate bioengineering approaches with vascular biology to understand how endothelial cell interacting with its environment and its role in the blood vessel regeneration and vascular disease processes, and to generate better designs in tissue engineering of vascular graft and microvasculature for organ regeneration.
Currently, the research programs in the laboratory focus on three goals: (1) To understand how biomechanical forces regulate vascular functions, and to identify targets associated with specific cellular phenotype in diseased blood vessels and develop technologies for targeted drug delivery and molecular imaging of those vasculatures; (2) To develop 3-D cell printing technology for vascular tissue engineering applications, such as engineering tissue structures with adequate vascular perfusion and designing optimal conditions for blood vessel regeneration; (3) To develop technology to differentiate stem cells toward vascular lineage in particular arterial and venous endothelial cells, and to apply them in tissue engineering of vascular graft. To accomplish these research programs, we will use multidisciplinary approaches combining various methods including engineering design, experimental and computational fluid mechanics, micro-fabrication, cellular and molecular techniques. There are excellent opportunities for collaboration with our BME faculty in the field of tissue engineering, biomaterials, cell mechanics as well as our colleagues at Albany Medical College.
cell and tissue engineering
3-D cell printing technology
Ph.D., Biomedical Engineering, Harvard Medical School - Massachusetts Institute of Technology
M.S., Biomechanics, Beijing University
B.S., Mechanical Engineering, Beijing University
Dai G, Gertler JP, Kamm RD, The effects of external compression on venous blood flow and tissue deformation in the lower leg. J Biomech Eng. 1999 Dec;121(6):557-64.
Dai G, Tsukurov O, Orkin RW, Abbott WM, Kamm RD, Gertler JP, An in vitro cell culture system to study the influence of external pneumatic compression on endothelial function. J Vasc Surg. 2000 Nov;32(5):977-87.
Casey PJ, Dattilo JB, Dai G, Albert JA, Tsukurov OI, Orkin RW, Gertler JP, Abbott WM, The effect of combined arterial hemodynamics on saphenous venous endothelial nitric oxide production. J Vasc Surg. 2001 Jun;33(6):1199-205.
Dai G, Tsukurov O, Chen M, Gertler JP, Kamm RD, Endothelial nitric oxide production during in vitro simulation of external limb compression. Am J Physiol, 2002 Jun;282(6):H2066-75.
Tschumperlin DJ, Dai G, Maly IV, Kikuchi T, Laiho LH, McVittie AK, Haley KJ, Lilly CM, So PT, Lauffenburger DA, Kamm RD, Drazen JM, Mechanotransduction through growth-factor shedding into the extracellular space. Nature, 2004 May 6;429(6987):83-6.
Dai G, Kaazempur-Mofrad MR, Natarajan S, Zhang Y, Vaughn S, Blackman BR, Kamm RD, Garcia-Cardena G, Gimbrone MA Jr., Distinct endothelial phenotypes evoked by arterial waveforms derived from atherosclerosis-susceptible and -resistant regions of human vasculature. Proc Natl Acad Sci. 2004 Oct 12;101(41):14871-6.
Parmar KM, Nambudiri V, Dai G, Larman HB, Gimbrone MA Jr, Garcia-Cardena G., Statins exert endothelial atheroprotective effects via the KLF2 transcription factor. J Biol Chem. 2005 Jul 22;280(29):26714-9.
Parmar KM, Larman HB, Dai G, Zhang Y, Wang ET, Moorthy SN, Kratz JR, Lin Z, Jain MK, Gimbrone MA Jr, Garcia-Cardena G. Integration of flow-dependent endothelial phenotypes by Kruppel-like factor 2. J Clin Invest. 2006 Jan;116(1):49-58.
Dai G, Wang ET, Zhang Y, Vaughn S, Garcia-Cardena G, Gimbrone MA Jr. Biomechanical forces in atherosclerosis-resistant vascular regions regulate endothelial redox balance via PI3K/Akt-dependent activation of Nrf2. Circulation Research, 2007 Sep 28;101(7):723-33.
Lee, Y.B., Polio, S., Lee, W., Dai, G., Menon, L., Carroll, R.S., and Yoo, S.S. 2010. Bio-printing of collagen and VEGF-releasing fibrin gel scaffolds for neural stem cell culture. Exp Neurol 223:645-652.
Zhao L, Lee VK, Yoo SS, Dai G, Intes X. The integration of 3-d cell printing and mesoscopic fluorescence molecular tomography of vascular constructs within thick hydrogel scaffolds. Biomaterials. 2012
Pandit, V., Zuidema, J.M., Venuto, K.N., Macione, J., Dai, G., Gilbert, R.J., and Kotha, S.P. "Evaluation of multifunctional polysaccharide hydrogels with varying stiffness for bone tissue engineering." Tissue Eng Part A 2013, 19:2452-2463.
Ozturk, M.S., Lee, V.K., Zhao, L., Dai, G., and Intes, X. "Mesoscopic fluorescence molecular tomography of reporter genes in bioprinted thick tissue." J Biomed Opt 2013, 18:100501.
Lee, V.K., Singh, G., Trasatti, J.P., Bjornsson, C., Tran, T.N., Xu, G., Yoo, S.S., Dai, G., and Karande, P. "Design and Fabrication of Human Skin by 3D Bioprinting." Tissue Eng Part C Methods 2013.
American Heart Association, Scientist Development Award, 2012
National Science Foundation, Faculty Early Career Award, 2014
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