Publications

1. Zafeiropoulus, S; Ahmed, U; Bekiaridou, A; … Puleo, C; Giannkoulas, G; Nicolls, M; Diamond B; Zanos, S. Ultrasound neuromodulation of an anti-inflammatory pathway at the spleen improves experimental pulmonary hypertension. Circulation Research. 2024 Jun 21;135(1):41-56. 
2. Khire, TS; Gao, W; Bales, B; Hsieh, K; Grossman, G; Park, DJM, et al, and Puleo, C. Rapid Minimum Inhibitory Concentration (MIC) Analysis using Lyophilized Reagent Beads in a Novel Multi-phase, Single-Vessel Assay. Antibiotics. 2023, 12(11)1641
3. Song, W; Jayaprakash, N; Salenkezhad, N; Puleo, C; … et al. Trans-spinal focused ultrasound suppresses spinal reflexes in healthy rats. Neuromodulation: Technology at the Interface, 2023, S1094-7159(23) 00649-9
4. J Ashe, J Graf, R Madhavan, K Wallace, V, Cotero, … C Puleo. Investigation of liver-targeted peripheral focused ultrasound stimulation (pFUS) and its effects on glucose homeostasis and insulin resistance in type 2 diabetes mellitus: a proof of concept, phase 1 trial; QJM: An International Journal of Medicine, 116(8), 2023, 667-685
5. S Zanos, D Ntiloudi, J Pellerito, R Ramdeo, … C, Puleo … et al.  Focused ultrasound neuromodulation of the spleen activates an anti-inflammatory response in humans. Brain Stimulation. 16(3), 703-711
6. Morton, C., Cotero, V., Ashe, J., Ginty, F., Puleo, C. Accelerating cutaneous healing in a rodent model of type II diabetes utilizing non-invasive focused ultrasound targeted at the spleen. Frontiers in Neuroscience, Online First, 2022.
7. Cotero, V., Graf, J., Miwa, … Puleo, C. Stimulation of the hepatoportal nerve plexus with focused ultrasound restores glucose homoeostasis in diabetic mice, rats and swine. Nature Biomed. Eng. 6, 683–705 (2022). 
8. Cotero, V., Ashe, J., Puleo, C. Therapeutic ultrasound modulates autonomic nerve pathways in diabetes. Nature Biomed. Eng. 6, 681–682 (2022).
9. Ahmed, U., Graf, J., Daytz, A., Yaipen, O., …, Puleo, C. Ultrasound neuromodulation of the spleen has time-dependent anti-inflammatory effect in a pneumonia model. Frontiers in Immunology, 13, 892086, 2022
10. T Datta-Chaudhuri, T Zanos, … ,Puleo, C., Pavlov, VA.,  Al-Abed, Y.,  Tracey, KJ. The Fourth Bioelectronic Medicine Summit “Technology Targeting Molecular Mechanisms”: current progress, challenges, and charting the future. Bioelectronic Medicine. 7 (1), 1-9, 2021
11. B Forsyth, P Torab, … , Puleo, C and Wong PK. A Rapid Single-Cell Antimicrobial Susceptibility Testing Workflow for Bloodstream Infections., Biosensors, 11(8), 288, 2021
12. Akhtar, K, Hirchstein, Z, Stefanelli, A, …, Puleo, C, Shin, D. Non-invasive peripheral focused ultrasound neuromodulation of the celiac plexus ameliorates symptoms in a rat model of inflammatory bowel disease. Experimental Physiology, 106 (4), 1038-1060, 2021
13. Huerta,TS, Devarajan, A, Tsaava, T, … Puleo, C, Ashe, J, Chavan, S. Targeted peripheral focused ultrasound stimulation attenuates obesity-induced metabolic and inflammatory dysfunctions. Scientific Reports,11 (1),1-12, 2021
14. Kaushik, AM, Hsieh,K, … Puleo, C, Carroll, KC, Liao, JC, Wang, TH. Droplet-based single-cell measurements of 16S rRNA enable integrated bacteria identification and pheno-molecular antimicrobial susceptibility testing from clinical samples in 30 minutes. Advanced Science, 8 (6), 2003419, 2021
15. Scherer, B, Surrette, C, Li, H, … Puleo, C. Digital electrical impedance analysis for single bacterium sensing and antimicrobial susceptibility testing. Lab on a Chip., 21, (6), 1073-1083, 2021
16. Cotero, V, Kao, TJ, Graf, J, ... Puleo, C. Evidence of long-range nerve pathways connecting and coordinating activity in secondary lymph organs., Bioelectronic medicine, 6(1),1-14, 2020
17. Gaddes, DE., Lee, P., … Puleo, C, … Wang, TH. Facile Coupling of Droplet Magnetofluidic-enabled Automated Sample Preparation for Digital Nucleic Acid Amplification Testing and Analysis. Analytical Chemistry, 92 (19), 13254-13261, 2020
18. Cotero, V, Miwa, H, … Puleo, C. Peripheral focused ultrasound neuromodulation (pFUS). Journal of Neuroscience Methods. 341, 108721, 2020
19. Puleo, C and Cotero, V. Noninvasive neuromodulation of peripheral nerve pathways using ultrasound and its current therapeutic implications. Cold Spring Harbor Perspectives in Medicine, 10 (2), a034215, 2020
20. Cotero, V, Graf, J, … Puleo, C. Peripheral focused ultrasound stimulation (pFUS): new competitor in pharmaceutical markets? SLAS Technology, 24 (4), 448-452, 2019
21. LI, H, Torab, P, … Puleo, C, Wong PK.  Adaptable microfluidic system for single-cell pathogen classification and antimicrobial susceptibility testing. PNAS, 116 (21), 10279, 10279, 2019
22. Gao, J, Li, H, Torab, P, Puleo, C, … Liao, JC, Wang, TH, Wong, PK. Nanotube assisted microwave electroporation for single cell pathogen identification and antimicrobial susceptibility testing. Nanomedicine: Nanotechnology, Biology and Medicine. 17, 246-253, 2019
23. Cotero, V, Fan, Y, … Puleo, CM. Noninvasive sub-organ ultrasound stimulation for targeted neuromodulation. Nature communications., 10 (1), 1-12, 2019
24. Chen, G, Dodson, B, Johnson, F, Hancu, I, Fiveland, E, Zhang, W, Galligan, C, Puleo, C …Tissue-susceptibility matched carbon nanotube electrodes for magnetic resonance imaging. Journal of Magnetic Resonance, 295, 72-79, 2018. 
25. Surrette, C, Scherer, B, … Puleo, CM. Rapid microbiology screening in pharmaceutical workflows. SLAS Technology, 23 (4), 387-394, 2018
26. CM Puleo, Davis, B, Smith, R. Enabling Technology in Cell-based Therapies: Scale-Up, Scale-Out, or Program in Place. SLAS Technology, 23 (4), 299-300, 2018
27. Galligan C, Nguyen, … Puleo, CM. High-capacity redox polymer electrodes: Applications in Molecular and Cellular Processing. SLAS Technology, 23 (4), 374-386, 2018
28. Gunasekaran, M, Chatterjee, PK, Shih, A, Imperato, GH, Addorisio, M, Puleo, C. … Tracey, KH, Chavan, SS. Immunization elicits antigen-specific antibody sequestration in dorsal root ganglia sensory neurons. Frontiers in Immunology. 9, 638, 2018.  
29. Chen, G, Dodson, B, Steffensen, SC, Harb, JN, Puleo, C. … Fabrication of high aspect ratio millimeter-tall free-standing carbon nanotube-based microelectrode arrays. ACS Biomaterials Science & Engineering, 4 (5), 1900-1907, 2018
30. Murray, C, Miwa, H, … Puleo, CM, and Di Carlo, D. Unsupervised capture and profiling of rare immune cells using multi-directional magnetic ratcheting. Lab on a Chip. 18 (16), 2396-24098, 2018. 
31. Surrette, C, Shoudy, D, … Puleo, CM. Microfluidic tissue mesodissection in molecular cancer diagnostics. SLAS Technology., 22 (4), 425-430, 2017
32. Galligan, C, Nichols, J, … Puleo, CM. Mesoscale blood cell sedimentation for processing milliliter sample volumes. Lab on a Chip., 15 (16), 3274-3277, 2015
33. Rane, TD, Zec, HC, Puleo, C, Lee, AP, Wang, TH. Droplet microfluidics for amplification-free genetic detection of single cells. Lab on a Chip, 12 (18), 3341-3347, 2012
34. Rane, TD, Puleo, CM, Liu, KJ, Zhang, Y, Lee, AP, Wang, TH. Counting single molecules in sub-nanolitre droplets. Lab on a Chip, 10 (2), 161-164, 2010
35. Nalayanda, DD, Puleo, C, Fulton, WB … An open-access microfluidic model for lung-specific functional studies at an air-liquid interface. Biomedical microdevices, 11 (5), 1081-1089, 2009
36. Puleo, CM, Wang, TH. Microfluidic means of achieving attomolar detection limits with molecular beacon probes. Lab on a Chip. 9 (8), 1065-1072, 2009.
37. Puleo, CM, Ambrose, WMI, Takezawa, T, Elisseeff, J, Wang, TH. Integration and application of vitrified collagen in multilayered devices for corneal microtissue culture. Lab on a Chip. 9 (22), 3221-3227, 2009
38. Zhang, Y, Bailey, V, Puleo, CM, Easwaran, H, … DNA methylation analysis on a droplet-in-oil PCR array. Lab on a Chip. 9 (8), 1059-1064, 2009
39. Yeh, HC, Puleo, CM, … Wang, TH. Tunable blinking kinetics of Cy5 for precise DNA quantification and single-nucleotide difference detection. Biophysical journal. 95 (2), 729-737, 2008
40. Puleo, CM, Yeh HC, Liu, KJ, Wang TH. Coupling confocal fluorescence detection and recirculating microfluidic control for single particle analysis in discrete nanoliter volumes. Lab on a Chip. 8 (5), 822-825, 2008
41. Puleo, CM, Yeh, HC, Wang TH. Applications of MEMS technologies in tissue engineering. Tissue Engineering, 13 (12), 2839-2854, 2007
42. Hwang, NS, Varghese, S, Puleo, C, Zhang, Z, Elisseeff, J. Morphogenetic signals from chondrocyte promote chondrogenic and osteogenic differentiation of mesenchymal stem cells. Journal of Cellular Physiology, 212 (2), 281-284, 2007
43. Nalayanda, DD, Puleo, CM, Fulton, WB, Wang, TH, Abdullah, F. Characterization of pulmonary cell growth parameters in a continuous perfusion microfluidic environment. Experimental Lung Research, 33 (6), 321-335, 2007
44. Yeh, HC, Puleo, CM, Lim, TC, Ho, YP, Giza, PE, Huang, RCC, Wang, TH. A microfluidic-FCS platform for investigation on the dissociation of Sp1-DNA complex by doxorubicin. Nucleic acids research, 34 (21), e144, 2006
45. Puleo, CM, Liu, J, Wang, TH. Pushing miRNA quantification to the limits: high-throughput miRNA gene expression analysis using single-molecule detection. Future Medicine, 1 (1), 123-127, 2006

Patents

1. Neuromodulation to target glucose transporter and/or incretin pathways. VE Cotero, CM Puleo, JF Graf. US Patent 12,029,577
2. Neuromodulation energy application technique. CM Puleo, JM Ashe, DA Shoudy, VE Cotero, JE Sabatini, US Patent 11,944,490
3. Neuromodulation technique. CM Puleo, VE Cotero. US Patent 11,938,348
4. Neuromodulation energy application techniques. CM Puleo, Y Zhao, K Wallace, Y Fan, J Ashe, D Shoudy. US Patent 11,911,634
5. Neuromodulation techniques. CM Puleo, L Kaanumalle, V Cotero, J Graf. US Patent 11,883,689.
6. Multibeam Neuromodulation Techniques. JF Graf, CM Puleo, JM Ashe, VE Cotero, DA Shoudy. US Patent 11,786,760.
7. Neuromodulation Techniques. CM Puleo, V Cotero. Ultrasound neuromodulation of a portion of a ganglia. US Patent 11,660,474
8. Noninvasive tissue displacement control and monitoring for neuromodulation. J Ashe, CM Puleo, et al. US Patent 11,633,630
9. Delivery of Therapeutic Neuromodulation. A Singanamalli, D Shoudy, J Ashe, CM Puleo. US Patent 11,602,331
10. Systems and methods for prescription and dosing of therapeutic stimuli using recorded guarantees. CM Puleo, B Beckman, V Abate, J Freer, D Vernooy, J Ashe. US Patent 11,551,796
11. Neuromodulation techniques for perturbation of physiological systems. CM Puleo, VE Cotero, JM Ashe, JF Graf., USPatent 11,426,610
12. High-capacity redox electrodes and their use in cell lysis. AJ Peter, TE Stecher, CP Galligan, CM Puleo, R Lenigk., USPatent 11,402,349
13. Neuromodulation techniques, CM Puleo, LS Kaanumalle, VE Cotero, JF Graf. US Patent 11,318,330
14. Neuromodulation energy application techniques, CM Puleo, Y Zhao, KD Wallace, Y Fan, JM Ashe, DA Shoudy, US Patent 11,235,178
15. Method and device for collection and amplification of circulating nucleic acids, EL Kvam, JR Nelson, GA Grossmann, RC Heller, EJ Finehout, CM Puleo, ...US Patent 11,142,758
16. Techniques for neuromodulation, CM Puleo, JM Ashe, VE Cotero, ME Marino, US Patent 11,040,199
17. Methods for electroelution of biomolecules, CM Puleo, JR Nelson, PMC Spooner, R Lenigk, NL Wood, L Zhu, ...US Patent 11,033,859
18. Neuromodulation techniques, CM Puleo, VE Cotero, US Patent 10,974,079
19. Systems and methods for rapidly sensing microbial metabolism, CM Puleo, CL Surrette, EL Kvam, SYH Go, F Chen, JR Nelson, ..., US Patent 10,787,695
20. Multimodal probe array, JM Ashe, KR Nagarkar, CM Puleo, CF Keimel, CP Galligan, Y Lin, ..., US Patent 10,602,950
21. Devices for separation of particulates, associated methods and systems, CM Puleo, CP Galligan, GA Grossman, EL Kvam, RS Duthie, KW Rigby, ..., US Patent 10,518,196
22. Extraction of materials from regions of interest in a sample, JR Nelson, W Gao, CM Puleo, TF Miller, CL Pitner, DA Shoudy, ..., US Patent 10,494,627
23. Methods for electroelution of biomolecules, CM Puleo, JR Nelson, PMC Spooner, R Lenigk, NL Wood, L Zhu, ..., US Patent 9,999,856
24. Detection of nucleic acid amplification in a porous substrate, EJ Finehout, CM Puleo, R Lenigk, JM Nichols, J Nelson, US Patent 9,714,447
25. Method and device for collection and amplification of circulating nucleic acids, EL Kvam, JR Nelson, GA Grossmann, RC Heller, EJ Finehout, CM Puleo, ..., US Patent 9,644,232
26. Extraction of materials from regions of interest in a sample, JR Nelson, W Gao, CM Puleo, TF Miller, CL Pitner, DA Shoudy, ..., US Patent 9,625,355
27. Systems for separation of particulates and associated methods and devices, CM Puleo, JL Davis, JM Nichols, US Patent 9,616,361
28. Methods for amplifying nucleic acids on substrates, JR Nelson, RS Duthie, CM Puleo, PMC Spooner, US Patent 9,593,368
29. Devices and systems for isolating biomolecules and associated methods thereof, JR Nelson, L Zhu, X Chen, CM Puleo, PMC Spooner, US Patent 9,399,986
30. Devices and systems for elution of biomolecules, CM Puleo, JR Nelson, PMC Spooner, R Lenigk, NL Wood, L Zhu, ...US Patent 9,333,463
31. Microfluidic system for high-throughput, droplet-based single molecule analysis with low reagent consumption, TH Wang, KJ Liu, CM Puleo, TD Rane, US Patent 9,284,601
32. Self-contained electroosmotic pump and method of making thereof, CM Puleo, R Lenigk, US Patent 9,199,201
33. Actuation of valves using electroosmotic pump, CM Puleo, CF Keimel, CP Galligan, US Patent 9,188,113
34. Electro-osmotic pump, CM Puleo, CF Keimel, X Chen, R Lenigk, CP Galligan, TF Miller, US Patent 9,103,331
35. Device and method for drying biological sample on substrate, CM Puleo, JR Nelson, PMC Spooner, R Lenigk, US Patent 9,063,041
36. Microfluidic solution for high-throughput, droplet-based single molecule analysis with low reagent consumption, JTH Wang, KJ Liu, CM Puleo, TD Rane, US Patent 8,637,301
37. Actuation of valves using electroosmotic pump, CM Puleo, CF Keimel, CP Galligan, US Patent 8,603,834

38. Devices and methods for batch processing magnetic bead assays, KA Shaikh, CM Puleo, J Xie, H Zeng, L Zhu, US Patent 8,449,838